Chapter 4Cloning

pg 52Restriction endonucleases

pg 54Vectors

pg 56Replica plating

pg 63Probes

pg 64cDNAs

pg 66RACE (Rapid amplification of cDNA ends)

pg 67PCR (Fig 4.15)

pg 69RT-PCR

pg 71Real-time PCR

pg 71Expression of cloned genes in prokaryotes

pg 72Expression vectors

pg 74His-tagging

pg 75Fusion proteins

pg 75Expression cloning (Fig. 4.22)

pg 76Expression of cloned genes in eukaryotes

pg 78Transformation, transfection

pg 78YACS, BACS, P1 vectors

pg 78Ti Plasmids & transgenic plants

Chapter 5

pg 84Electrophoresis

pg 88Ion-Exchange Chromatography

pg 88Gel Filtration Chromatography

pg 89Affinity Chromatography

pg 90Labeling (5.2 Labeled Tracers)

Autoradiography, Phosphorimaging, Liquid Scintillation

Counting, Non-radioactive tracers

pg 93Southern Blotting

pg 94DNA fingerprinting

pg 96In situ hybridization

pg 97Western Blots

pg 97DNA sequencing

pg 101Restriction mapping

pg 105Site-directed mutagenesis

pg 106Northern blots

pg 107S1 Mapping

pg 109Primer extension

pg 110Run-off Transcription

pg 112Nuclear Run-On transcription

pg 113Reporter gene transcription

pg 115Filter binding of DNA protein mixtures

pg 115Gel mobility shift

pg 116DNase Footprinting

pg 118DMS Footprinting

pg 119SELEX

pg 120Knock outs (Fig 5.40) & transgenic animals