Myler LR, Soniat MM, Zhang X, Deshpande RA, Paull TT, Finkelstein IJ. (2019) Purification and Biophysical Characterization of the Mre11-Rad50-Nbs1 Complex. Methods Mol Biol. 2004:269-287. [pubmed]


Lee, J.-H., Mand, M.R., Kao, C.-H., Zhou, Y., Ryu, S.W., Richards, A.L., Coon, J.J., and Paull, T.T. (2018). ATM directs DNA damage responses and proteostasis via genetically separable pathways. Science Signaling 11, pii: eaan5598. [pubmed]

Makharashvili, N., Arora, S., Yin, Y., Fu, Q., Wen, X., Lee, J-H., Kao, C-H., Leung, J.W., Miller, K.M., and Paull, T.T. (2018). Sae2/CtIP prevents R-loop accumulation in eukaryotic cells. Elife 7. pii: e42733. [pubmed]


Hewitt, S.L., Wong, J.B., Lee, J.H., Nishana, M., Chen, H., Coussens, M., Arnal, S.M., Blumenberg, L.M., Roth, D.B., Paull, T.T., and Skok, J.A. (2017). The Conserved ATM Kinase RAG2-S365 Phosphorylation Site Limits Cleavage Events in Individual Cells Independent of Any Repair Defect. Cell Reports 21, 979-993. [pubmed]

Arora, S., Deshpande, R.A., Budd, M., Campbell, J., Revere, A., Zhang, X., Schmidt, K.H., and Paull, T.T. (2017) Genetic separation of Sae2 nuclease activity from Mre11 nuclease functions in budding yeast. Molecular and Cellular Biology 37: e00156-17. [pubmed]

Myler, L.R., Gallardo, I.F., Deshpande, R.A., Gonzalez, X.B., Kim, Y., Paull, T.T., and Finkelstein, I.J. (2017). Single-molecule imaging reveals how Mre11-Rad50-Nbs1 initiates DNA break repair. Molecular Cell 67, 891-898. [pubmed]

Deshpande, R., Lee, J.-H., and Paull, T.T. (2017). Rad50 ATPase activity is regulated by DNA ends and requires coordination of both active sites. Nucleic Acids Research 45, 5255-5268. [pubmed]

Zhou, Y., Lee, J.H., Jiang, W., Crowe, J.L., Zha, S., and Paull, T.T. (2017). Regulation of the DNA Damage Response by DNA-PKcs Inhibitory Phosphorylation of ATM. Molecular Cell 65, 91-104. [pubmed]


Deshpande, R.A., Lee, J.H., Arora, S., and Paull, T.T. (2016). Nbs1 Converts the Human Mre11/Rad50 Nuclease Complex into an Endo/Exonuclease Machine Specific for Protein-DNA Adducts. Molecular Cell 64, 593-606. [pubmed]

Hoa, N.N., Shimizu, T., Zhou, Z.W., Wang, Z.Q., Deshpande, R.A., Paull, T.T., Akter, S., Tsuda, M., Furuta, R., Tsutsui, K., et al. (2016). Mre11 Is Essential for the Removal of Lethal Topoisomerase 2 Covalent Cleavage Complexes. Molecular Cell 64, 580-592. [pubmed]

Myler, L.R., Gallardo, I.F., Zhou, Y., Gong, F., Yang, S.H., Wold, M.S., Miller, K.M., Paull, T.T.*, and Finkelstein, I.J*. (2016). Single-molecule imaging reveals the mechanism of Exo1 regulation by single-stranded DNA binding proteins. Proceedings of the National Academy of Sciences of the United States of America 113, E1170-1179. * co-contributing [pubmed]

Broderick, R., Nieminuszczy, J., Baddock, H.T., Deshpande, R.A., Gileadi, O., Paull, T.T., McHugh, P.J., and Niedzwiedz, W. (2016). EXD2 promotes homologous recombination by facilitating DNA end resection. Nat Cell Biol. 18, 271-280. [pubmed]


Zhang, J., Tripathi, D., Jing, J., Alexander, A., Kim, J., Powell, R., Dere, R., Tait-Mulder, J., Lee, J-H., Paull, T.T., Pandita, R.K., Charaka, V., Pandita, T., Kastan, M., and Walker, C. (2015). ATM Functions at the Peroxisome to Induce Pexophagy in Response to ROS. Nature Cell Biol. 17:1259-1269. [pubmed]

Sarangi, P., Steinacher, R., Altmannova, V., Fu, Q., Paull, T.T., Krejci, L., Whitby, M.C., and Zhao, X. (2015). Sumoylation influences DNA break repair partly by increasing the solubility of a conserved end resection protein. PLoS Genetics 11, e1004899. [pubmed]

Hoa, N.N., Kobayashi, J., Omura, M., Hirakawa, M., Yang, S.H., Komatsu, K., Paull, T.T., Takeda, S., and Sasanuma, H. (2015). BRCA1 and CtIP Are Both Required to Recruit Dna2 at Double-Strand Breaks in Homologous Recombination. PloS One 10, e0124495. [pubmed]

Zhou, Y., and Paull, T.T. (2015). Direct measurement of single-stranded DNA intermediates in mammalian cells by quantitative polymerase chain reaction. Anal Biochem 479, 48-50. [pubmed]

Parameswaran, B., Chiang, H.C., Lu, Y., Coates, J., Deng, C.X., Baer, R., Lin, H.K., Li, R., Paull, T.T., and Hu, Y. (2015). Damage-induced BRCA1 phosphorylation by Chk2 contributes to the timing of end resection. Cell Cycle 14, 437-448. [pubmed]


Zhou, Y., and Paull, T.T. (2014). Quantitation of DNA double-strand break resection intermediates in human cells. Nucleic Acids Research 42(3):e19. [pubmed]

Lee, J.-H., Guo, Z., Myler, L.R., Zheng, S., and Paull, T.T. (2014). Direct activation of ATM by resveratrol under oxidizing conditions. PloS ONE 9(6):e97969. [pubmed]

Makharashvili, N., Tubbs, A.T., Yang, S.H., Wang, H., Barton, O., Zhou, Y., Deshpande, R.A., Lobrich, M., Sleckman, B.P., Wu, X., and Paull, T.T. (2014). Catalytic and noncatalytic roles of the CtIP endonuclease in double-strand break end resection. Mol. Cell 54(6): 1022-1023. [pubmed]

Deshpande, R., Williams, G.J., Limbo, O., Williams, R.S., Kuhnlein, J., Lee, J.-H., Classen, S., Guenther, G., Russell, P., Tainer, J.A., and Paull, T.T.(2014). ATP-driven Rad50 conformations regulate DNA tethering, end resection, and ATM checkpoint signaling. EMBO J.33(5):482-500. [pubmed]


Fu, Q., Chow, J, Bernstein, K.A., Makharashvili, N., Arora, S., Lee, C-F., Person, M., Rothstein, R., and Paull, T.T. (2013) Phosphorylation-regulated transitions in oligomeric state control the activity of the Sae2 DNA repair enzyme. Mol. Cell. Biol. 34(5):778-793. [pubmed]

Cannon, B., Kuhnlein, J., Yang, S.H., Cheng, A., Schindler, D., Stark, J.M., Russell, R., and Paull, T.T. (2013). Visualization of local DNA unwinding by Mre11-Rad50-Nbs1 using single-molecule FRET. Proceedings of the National Academy of Sciences of the United States of America 110(47):18868-73. [pubmed]

Zhou, Y., and Paull, T.T. (2013). DNA-dependent Protein Kinase regulates DNA end resection in concert with Mre11-Rad50-Nbs1 (MRN) and Ataxia-Telangiectasia-Mutated (ATM). J. Biol. Chem. 288(52):37112-25. [pubmed]

Bowen, C., Ju, J.H., Lee, J-H., Paull, T.T., and Gelmann, E.P. (2013). Functional Activation of ATM by the Prostate Cancer Suppressor NKX3.1. Cell Reports 4, 516-529. [pubmed]

Lee, J-H., Mand, M.R., Deshpande, R.A., Kinoshita, E., Yang, S-H., Wyman, C., and Paull, T.T. (2013). Ataxia Telangiectasia-Mutated (ATM) Kinase Activity Is Regulated by ATP-driven Conformational Changes in the Mre11/Rad50/Nbs1 (MRN) Complex. J Biol Chem 288, 12840-12851. [pubmed]

Yang, S-H., Zhou, R., Campbell, J., Chen, J., Ha, T., and Paull, T.T. (2013) The SOSS1 single-stranded DNA binding complex promotes DNA end resection in concert with Exo1.  EMBO J 32(1):126-39. [pubmed]


Daniel, J.A., Pellegrini, M., Lee, B.S., Guo, Z., Filsuf, D., Belkina, N.V., You, Z., Paull, T.T., Sleckman, B.P., Feigenbaum, L., Nussenzweig, A. (2012) Loss of ATM kinase activity leads to embryonic lethality in mice. J Cell Biol 198(3): 295-304. [pubmed]

Gatei, M., Jakob, B., Chen, P., Kijas, A.W., Becherel, O.J., Gueven, N., Birrell, G., Lee, J.H., Paull, T.T., Lerenthal, Y., Fazry, S. Taucher-Scholz, G., Kalb, R., Schindler, D., Waltes, R., Dork, T., and Lavin, M. F. (2011). ATM protein-dependent phosphorylation of Rad50 protein regulates DNA repair and cell cycle control. J Biol Chem 286, 31542-31556. [pubmed]


Della-Maria, J., Zhou, Y., Tsai, M.S., Kuhnlein, J., Carney, J., Paull, T.T., Tomkinson A (2011) hMre11/hRad50/Nbs1 and DNA ligase III{alpha}/XRCC1 act together in an alternative non-homologous end joining pathway. J Biol Chem. 286(39):33845-33853. [pubmed]


Nicolette, M.L., Lee, K., Guo, Z., Rani, M., Chow, J.M., Lee, S.E. and Paull, T.T. (2010) A direct role for Mre11/Rad50/Xrs2 and Sae2 in 5’ strand resection of DNA double-strand breaks. Nature Structural and Molecular Biology 17(12):1478-85. [pubmed]

Guo, Z., Kozlov, S., Lavin, M.F., Person, M.D., and Paull, T.T. (2010) ATM Activation by Oxidative Stress. Science 330:517-521. [pubmed]

Shim, E.Y., Chung, W.H., Nicolette, M.L., Zhang, Y., Davis, M., Zhu, Z., Paull, T.T., Ira, G., and Lee, S.E. (2010). Saccharomyces cerevisiae Mre11/Rad50/Xrs2 and Ku proteins regulate association of Exo1 and Dna2 with DNA breaks. EMBO J 29, 3370-3380. [pubmed]

Lee, J.H., Goodarzi, A.A., Jeggo, P.A., and Paull, T.T. (2010) 53BP1 promotes ATM activity through direct interactions with the MRN complex. EMBO J. 29:574-585. [pubmed]


Hopkins, B.B., and Paull, T.T. (2008) The P. furiosus Mre11/Rad50 complex promotes 5’ strand resection at a DNA double-strand break. Cell 135: 250-260. [pubmed]

Dupre, A., Boyer-Chatenet, L., Sattler, R.M., Modi, A.P., Lee, J.H., Nicolette, M.L., Kopelovich, L., Jasin, M., Baer, R., Paull, T.T., and Gautier, J. (2008). A forward chemical genetic screen reveals an inhibitor of the Mre11-Rad50-Nbs1 complex. Nat. Chem. Biol. 4, 119-125. [pubmed]


Lengsfeld, B.M., Rattray, A.J., Bhaskara, V., Ghirlando, R., and Paull, T.T. (2007). Sae2 is an endonuclease that processes hairpin DNA cooperatively with the Mre11/Rad50/Xrs2 complex. Mol. Cell 28: 638-651. [pubmed]

Bhaskara, V., Dupre, A., Lengsfeld, B., Hopkins, B. B., Chan, A., Lee, J. H., Zhang, X., Gautier, J., Zakian, V. A., and Paull, T. T. (2007). Rad50 Adenylate Kinase Activity Regulates DNA Tethering by Mre11/Rad50 complexes. Mol. Cell 25: 647-661. [pubmed]


Zhang, X. and Paull, T.T. (2005) The Mre11/Rad50/Xrs2 complex and non-homologous end-joining of incompatible ends in S. cerevisiae. DNA Repair 4: 1281-1294. [pubmed]

Lee, J.H. and Paull, T.T. (2005) ATM activation by DNA double-strand breaks through the Mre11-Rad50-Nbs1 complex. Science, 308: 551-554. [pubmed]


Lee, J.-H. and Paull, T.T. (2004). The Mre11/Rad50/Nbs1 complex directly promotes ATM kinase activity. Science 304: 93-96. [pubmed]

Moncalian, G., Lengsfeld, B., Bhaskara, V., Hopfner, K.P., Karcher, A., Alden, E., Tainer, J.A. and Paull, T.T. (2004) The rad50 signature motif: essential to ATP binding and biological function. J Mol. Biol. 335: 937-951. [pubmed]


Lee, J.-H., Ghirlando, R., Bhaskara, V., Hoffmeyer, M.R., Gu, J. and Paull, T.T. (2003) Regulation of Mre11/Rad50 by Nbs1: effects on nucleotide-dependent DNA binding and association with ATLD mutant complexes. J. Biol. Chem. 278: 45171-45181. [link]