Compact shRNA Design Tool

Sullivan Lab. Department of Molecular Biosciences. The University of Texas at Austin.

Enter Target Sequence

Output Design

Options

Promoter Architecture

BLV-like

Cutoffs

Max Hits:
Max GC %:
Min GC %:
Exclude Internal Terminator-like
Exclude Repetitive Sequences
Maintain A Box 2
Require 5' U
Require 3' Pairing
Add 5' Flanking Sequence

Generate shRNA

Cite: Burke et al. "Expression of short hairpin RNAs using compact retroviral miRNA gene architecture" In Prep.