Chapter 1 A Brief History

Chapter 2 The Molecular Nature of Genes

Chapter 3 An Introduction to Gene Function

Review material. Read it all.

Readings to match the lectures

Chapter 4 & 5.

These chapters contain methods that are important in subsequent chapters. Many of you are familiar with these. Please skim through the chapters and familiarize your self with which methods are explained. If you run into such a method in a future chapter and if you are not sure how the technique works then I want you to flip back to these chapters and review the technique. Here is a list of techniques discussed in these chapters.

Chapter 5. Methods. I will mention introduce these.

***pg 84    Ch 5 Electrophoresis

***pg 88    Ch 5 Ion-Exchange Chromatography

***pg 88    Ch 5 Gel Filtration Chromatography

***pg 90    Ch 5 Labeling

***pg 107  Ch 5 S1 mapping

***pg 107Ch 5 S1 Mapping Ch 10

***pg 109Ch 5 Primer extension Ch 10

***pg 110Ch 5 Run-off Transcription Ch 10

Chapter 10. Eukaryotic RNA polymerases and their promoters

Study pg 250-256

***

Read pg 256 Core subunits->257

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Study pg 257->Heterogeneity of the Rpb1 Subunit->258 The Three-dimensional structure of RNA Polymerase II

Read pg 258 The Three-dimensional structure of RNA Polymerase II ->266 10.2 Promoters

Study pg 266 10.2 Promoters ->end

Chapter 11. General transcription factors in eukaryotes

Study pg 280->end of chapter

***pg 115    Ch 5 Gel mobility shift Ch 11

***pg 116    Ch 5 DNase footprinting Ch11

Chapter 12. General transcription factors in eukaryotes

Study pg 333->end of chapter.

In the reading assignments below, “Study” means to read it at least 3 times and to try to understand everything and to memorize the terms. However, “Read” means to read it once and have a general idea of what it says. Sometimes, to understand a “Study” portion, you will need an idea that was presented in a “Read” assignment.