Macdonald Lab

Macdonald, PM, Ingham, P and Struhl, G (1986). Isolation, structure and expression of even-skipped: a second pair-rule gene of Drosophila containing a homeobox. Cell 47, 721-734.

Macdonald, PM and Struhl, G (1986). A molecular gradient in early Drosophila embryos and its role in specifying the body pattern. Nature 324, 537-545.

Lawrence, PA, Johnston, P, Macdonald, P and Struhl, G (1987). Borders of parasegments in Drosophila embryos are delimited by the fushi tarazu and even-skipped genes. Nature 328, 440-442.

Macdonald, PM and Struhl, G (1988). Cis-acting sequences responsible for anterior localization of bicoid mRNA in Drosophila embryos. Nature 336, 595-598.

Goto, T, Macdonald, P and Maniatis, T (1989). Early and late periodic patterns of even skipped expression are controlled by distinct regulatory elements that respond to different spatial cues. Cell 57, 413-422.

Struhl, G, Struhl, K and Macdonald, PM (1989). The gradient morphogen bicoid is a concentration-dependent transcriptional activator. Cell 57, 1259-1273.

Macdonald, PM (1990). bicoid mRNA localization signal: Phylogenetic conservation of function and RNA secondary structure. Development 110, 161-171. [Abstract]

Kim-Ha, J, Smith, JL and Macdonald, PM (1991). oskar mRNA is localized to the posterior pole of the Drosophila oocyte. Cell 66, 23-35. [Abstract]

Macdonald, PM, Luk, SK-S and Kilpatrick, M (1991). Protein encoded by the exuperantia gene is concentrated at sites of bicoid mRNA accumulation in Drosophila nurse cells, but not in oocytes or embryos. Genes & Development 5, 2455-2466. [Abstract]

Macdonald, PM (1992). The Drosophila pumilio gene: an unusually long transcription unit and an unusual protein. Development 114, 221-232. [Abstract]

Smith, JL, Wilson, JE and Macdonald, PM (1992). Overexpression of oskar directs ectopic activation of nanos and presumptive pole cell formation in Drosophila embryos. Cell 70, 849-859. [Abstract]

Macdonald, PM (1992). The means to the ends: localization of maternal messenger RNAs. Seminars in Developmental Biology 3, 413-424. [Abstract]

Wilson, J and Macdonald, PM (1993). Formation of the germ line in Drosophila. Current Opinion in Genetics & Development 3, 562-565. [Abstract]

Macdonald, PM, Kerr, K, Smith, JL and Leask, A (1993). RNA regulatory element BLE1 directs the early steps of bicoid mRNA localization. Development 118, 1233-1243. [Abstract]

Kim-Ha, J, Webster, P, Smith, JL and Macdonald, PM (1993). Multiple RNA regulatory elements mediate distinct steps in localization of oskar mRNA. Development 119, 169-178. [Abstract]

Luk, SK-S, Kilpatrick, M, Kerr, K and Macdonald, PM (1994). Components acting in localization of bicoid mRNA are conserved among Drosophila species. Genetics 137, 521-530. [Abstract]

Webster, PJ, Suen, J and Macdonald, PM (1994). Drosophila virilis oskar transgenes direct body patterning but not pole cell formation or maintenance of mRNA localization in D. melanogaster. Development 120, 2027-2037. [Abstract]

Kim-Ha, J, Kerr, K, and Macdonald, PM (1995). Translational regulation of oskar mRNA by bruno, an ovarian RNA binding protein, is essential. Cell 81, 403-412. [Abstract]

Macdonald, PM, Leask, A, and Kerr, K (1995). exl protein specifically binds BLE1, a bicoid mRNA localization element, and is required for one phase of its activity. Proceedings of the National Academy of Science 92, 10787-10791. [Abstract]

Wilson, JE, Connell, JE, and Macdonald, PM (1996). aubergine enhances oskar translation in the Drosophila ovary. Development 122, 1631-1639. [Abstract]

Macdonald, PM and Smibert, CA (1996). Translational regulation of maternal mRNAs. Current Opinion in Genetics & Development 6, 403-407. [Abstract]

Smibert, CA, Wilson, JE, Kerr, K and Macdonald, PM (1996). smaug protein represses translation of unlocalized nanos mRNA in the Drosophila embryo. Genes & Development 10, 2600-2609. [Abstract]

Wilson, JE, Connell, JE, Schlenker, JD and Macdonald, PM (1996). Novel genetic screen for genes involved in posterior body patterning in Drosophila. Developmental Genetics 19, 199-209. [Abstract]

Webster, PJ and Macdonald, PM (1997). Screening an expression library for RNA-binding proteins using a solution-based assay. In Methods in Molecular Biology: RNA-Protein Interaction Protocols,, (ed. S.R. Haynes), The Humana Press Inc., Totowa, NJ.

Webster, PJ, Liang, L, Berg, CA, Lasko, P and Macdonald, PM (1997). Translational repressor bruno plays multiple roles in development and is widely conserved. Genes & Development 11, 2510-2521. [Abstract]

Macdonald, PM and Kerr, K (1997). Redundant RNA recognition events in bicoid mRNA localization. RNA 3, 1413-1420. [Abstract]

Dubowy, J and Macdonald, PM (1998). Localization of mRNAs to the oocyte is common in Drosophila ovaries. Mechanisms of Development 70, 193-195. [Abstract]

Macdonald, PM and Kerr, K (1998). Mutational analysis of an RNA recognition element that mediates localization of bicoid mRNA. Molecular and Cellular Biology 18, 3788-3795. [Abstract]

Arn, EA and Macdonald, PM (1998). Motors driving mRNA localization: new insights from in vivo imaging. Cell 95, 151-154.

Lie, YS and Macdonald, PM (1999). Apontic binds the translational repressor Bruno and is implicated in regulation of oskar mRNA translation. Development 126, 1129-1138. [Abstract]

Lie, YS and Macdonald, PM (1999b). Translational regulation of oskar mRNA occurs independent of the cap and poly(A) tail in Drosophila ovarian extracts. Development 126, 4989-4996. [Abstract]

Smibert, CS, Lie, YS, Shillinglaw, W, Henzel, WJ and Macdonald, PM (1999). Smaug, a novel and conserved protein, contributes to repression of nanos mRNA translation in vitro. RNA 5, 1535-1547. [Abstract]

Lie, YS and Macdonald, PM (2000) In vitro translation of exogenous mRNAs in extracts prepared from Drosophila ovaries and embryos. Biochem Biophys Res Comm 270, 473-481. [Abstract]

Macdonald, PM Diversity in translational regulation. (2001) Current Opinion in Cell Biology 13, 326-331. [Abstract]

Mancebo, R., Zhou, X., Shillinglaw, W., Henzel, W. and Macdonald, P.M. (2001) BSF binds specifically to the bicoid mRNA and contributes to its stabilization. Molecular and Cellular Biology 21, 3462-3471. [Abstract]

Harris, AN and Macdonald, PM (2001) aubergine encodes a Drosophila polar granule component required for pole cell formation and related to eIF2C. Development 128, 2823-2832. [Abstract]

Arn, EA and Macdonald, PM (2001) RNA localization goes direct. Direct injection of RNA provides a new view of localization during Drosophila oogenesis. Developmental Cell 1, 155-156.

Arn, EA, Cha, BJ, Theurkauf, WE and Macdonald, PM (2003) Recognition of a bicoid mRNA localization signal by a protein complex containing Swallow, Nod and RNA binding proteins. Developmental Cell 4, 41-51. [Abstract]

Snee, MJ and Macdonald, PM (2004) Live imaging of nuage and polar granules: evidence against a precursor-product relationship, and a novel role for Oskar in stabilization of polar granule components. Journal of Cell Science 117, 2109-2120. [Abstract]

Macdonald, PM (2004) Translational Regulation: A Cup Half Full. Current Biology 14, R282-R283. [Abstract]

Braat, AK, Yan, N, Arn, E, Harrison, D and Macdonald, PM (2004) Localization-dependent Oskar protein accumulation: control after the initiation of translation. Developmental Cell 7, 125-131. [Abstract]

Yan N and Macdonald PM (2004) Genetic interactions of Drosophila melanogaster arrest reveal roles for translational repressor Bruno in accumulation of Gurken and activity of Delta. Genetics 168, 1433-1442. [Abstract]

Snee MJ, Arn EA, Bullock SL and Macdonald PM (2005) Recognition of the bcd mRNA localization signal in Drosophila embryos and ovaries. Molecular and Cellular Biology 25, 1501-1510. [Abstract]

Macdonald PM (2005) Translational repression by Bicoid: competition for the cap. Cell 121, 321-322.

Burgler C and Macdonald PM (2005) Prediction and verification of microRNA targets by MovingTargets, a highly adaptable prediction method. BMC Genomics 6, 88. [Abstract]

Geng C and Macdonald PM (2006) Imp associates with Squid and Hrp48 and contributes to localized expression of gurken. Molecular and Cellular Biology 26, 9508-9516. [Abstract]

Snee MJ, Harrison D, Yan N and Macdonald PM (2007) A late phase of Oskar accumulation is crucial for posterior patterning of the Drosophila embryo, and is blocked by ectopic expression of Bruno. Differentiation 75, 246-255. [Abstract]

Jones JR and Macdonald PM (2007) Oskar controls morphology of polar granules and nuclear bodies in Drosophila. Development 134, 233-236. [Abstract]

Snee MJ, Benz D, Jen J and Macdonald PM (2008) Two distinct domains of Bruno bind specifically to the oskar mRNA. RNA Biology 5, 49-57.

Reich J, Snee MJ and Macdonald PM (2009) miRNA-dependent translational repression in the Drosoophila ovary. PLosS ONE 4, e4669.

Snee MJ and Macdonald PM (2009) Bicaudal C and Trailer hitch have similar roles in gurken mRNA localization and cytoskeletal organization. Developmental Biology 328, 434-444.

Snee MJ and Macdonald PM (2009) Dynamic organization and plasticity of sponge bodies. Developmental Dynamics 238, 918-930.

Lyon AM, Reveal BS, Macdonald PM and Hoffman DW (2009) Bruno protein contains an expanded RNA recognition motif. Biochemistry 48, 12202-12212.

Reveal B, Yan N, Snee MJ, Pai C-I, Gim Y and Macdonald, PM (2010) BREs mediate both repression and activation of oskar mRNA translation and act in trans. Developmental Cell 18, 496-502.

Macdonald PM (2011) mRNA localization: assembly of transport complexes and their incorporation into particles. Current Opinion in Genetics and Development 21, 407-413. [Abstract]

Reveal B, Garcia C, Ellington A and Macdonald PM (2011) Multiple RNA binding domains of Bru confer recognition of diverse binding sites for translational repression. RNA Biology 8, 1047-1060. [Abstract]

Kim G, Pai C-I, Sato, K, Person, MD, Nakamura A and Macdonald PM (2015) Region-specific activation of oskar mRNA translation by inhibition of Bruno-mediated repression. PLoS Genetics 11:2, e100499 doi 10.1371/journal.pgen.1004992 [Abstract]

Kanke M, Jambor H, Reich J, Marches B, Gstir R, Ryu YH, Ephrussi A and Macdonald PM (2015) oskar RNA plays multiple noncoding roles to support oogenesis and maintain integrity of the germline/soma distinction. RNA 21, 1096-1109. [Abstract]

Kanke M and Macdonald PM (2015) Translational activation of oskar mRNA: reevaluation of the role and importance of a 5' regulatory element. PLoS One 10:5, e0125849 doi 10.1371/journal.pone.0125849.

Jones J and Macdonald PM (2015) Neurl4 contributes to germ cell formation and integrity in Drosophila. Biology Open doi 10.1242/bio.012351.

Ryu YH and Macdonald PM (2015) RNA Sequences Required for the Noncoding Function of oskar RNA also Mediate Regulation of Oskar Protein Expression by Bicoid Stability Factor. Developmental Biology 407, 211-223.

Macdonald PM, Kanke M and Kenny, A (2016) Community Effects in Regulation of Translation. eLife 2016;5:e10965.

Ryu YH, Kenny A, Gim Y, Snee M and Macdonald PM (2017) Multiple cis-acting signals, some weak by necessity, collectively direct robust transport of oskar mRNA to the oocyte. Journal of Cell Science 130, 3060-3071.