Links to sites provided by Leica Microsystems:

Download a pdf Leica manual for the SP5 Confocal
Download a slide presentation showing the components of the 2P
Download a pdf from Leica with slides explaining confocal microscopy

Download Leica information from a Stanford site

Leica New User Guide
Use of the FRAP wizard
Avoiding cross-talk between different fluorophores
Use of lambda scan (to scan through a series of wavelengths)
Use of viewer tools, orthogonal sectioning, autoscaling, histogram
Scanning a region of interest
How to do a sequential scan
How to correct for intensity loss during Z stack acquisition
Time Lapse
Dye separation

Other core facility sites with information on confocal/2P microscopy:

Cellular Imaging Core Facility, University of Arizona
Guide for FRAP using “FRAP wizard” from Univ. Washington
Guide for FRAP from University of Edinburgh

If you find a site that is particularly useful to you and you think it might be useful to others, send an email to Wesley Thompson and we will see about getting it listed here.